Publications 2019 – 2020
Publications 2019 – 2020
1. Sasnauskas G, Siksnys V. CRISPR adaptation from a structural perspective. Curr Opin Struct Biol. 2020 Dec;65:17-25. doi: 10.1016/j.sbi.2020.05.015. Epub 2020 Jun 20.
PMID: 32570107
2. Songailiene I, Juozapaitis J, Tamulaitiene G, Ruksenaite A, Šulčius S, Sasnauskas G, Venclovas Č, Siksnys V. HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. Mol Cell. 2020 Dec 17;80(6):955-970.e7. doi: 10.1016/j.molcel.2020.11.034. Epub 2020 Dec 7.
PMID: 33290744
3. Gasiunas G, Young JK, Karvelis T, Kazlauskas D, Urbaitis T, Jasnauskaite M, Grusyte MM, Paulraj S, Wang PH, Hou Z, Dooley SK, Cigan M, Alarcon C, Chilcoat ND, Bigelyte G, Curcuru JL, Mabuchi M, Sun Z, Fuchs RT, Schildkraut E, Weigele PR, Jack WE, Robb GB, Venclovas Č, Siksnys V. A catalogue of biochemically diverse CRISPR-Cas9 orthologs. Nat Commun. 2020 Nov 2;11(1):5512. doi: 10.1038/s41467-020-19344-1.
PMID: 33139742
4. Makarova KS, Timinskas A, Wolf YI, Gussow AB, Siksnys V, Venclovas Č, Koonin EV. Evolutionary and functional classification of the CARF domain superfamily, key sensors in prokaryotic antivirus defense. Nucleic Acids Res. 2020 Sep 18;48(16):8828-8847. doi: 10.1093/nar/gkaa635.
PMID: 32735657
5. Smalakyte D, Kazlauskiene M, F Havelund J, Rukšėnaitė A, Rimaite A, Tamulaitiene G, Færgeman NJ, Tamulaitis G, Siksnys V. Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains. Nucleic Acids Res. 2020 Sep 18;48(16):9204-9217. doi: 10.1093/nar/gkaa634.
PMID: 32766806
6. Fricke T, Smalakyte D, Lapinski M, Pateria A, Weige C, Pastor M, Kolano A, Winata C, Siksnys V, Tamulaitis G, Bochtler M. Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish.
CRISPR J. 2020 Aug;3(4):299-313. doi: 10.1089/crispr.2020.0032.
PMID: 32833532
7. Mullally G, van Aelst K, Naqvi MM, Diffin FM, Karvelis T, Gasiunas G, Siksnys V, Szczelkun MD. 5' modifications to CRISPR-Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage.
Nucleic Acids Res. 2020 Jul 9;48(12):6811-6823. doi: 10.1093/nar/gkaa477.
PMID: 32496535
8. Karvelis T, Bigelyte G, Young JK, Hou Z, Zedaveinyte R, Budre K, Paulraj S, Djukanovic V, Gasior S, Silanskas A, Venclovas Č, Siksnys V. PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage. Nucleic Acids Res. 2020 May 21;48(9):5016-5023. doi: 10.1093/nar/gkaa208.
PMID: 32246713
9. Makarova KS, Wolf YI, Iranzo J, Shmakov SA, Alkhnbashi OS, Brouns SJJ, Charpentier E, Cheng D, Haft DH, Horvath P, Moineau S, Mojica FJM, Scott D, Shah SA, Siksnys V, Terns MP, Venclovas Č, White MF, Yakunin AF, Yan W, Zhang F, Garrett RA, Backofen R, van der Oost J, Barrangou R, Koonin EV. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants. Nat Rev Microbiol. 2020 Feb;18(2):67-83. doi: 10.1038/s41579-019-0299-x. Epub 2019 Dec 19.
PMID: 31857715
10. Karvelis T, Young JK, Siksnys V. A pipeline for characterization of novel Cas9 orthologs. Methods Enzymol. 2019;616:219-240. doi: 10.1016/bs.mie.2018.10.021. Epub 2018 Dec 27.
PMID: 30691644
11. Songailiene I, Rutkauskas M, Sinkunas T, Manakova E, Wittig S, Schmidt C, Siksnys V, Seidel R. Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length. Cell Rep. 2019 Sep 17;28(12):3157-3166.e4. doi: 10.1016/j.celrep.2019.08.033.
PMID: 31533038
12. Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB. Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System. Mol Cell. 2019 Jul 11;75(1):90-101.e5. doi: 10.1016/j.molcel.2019.04.020. Epub 2019 May 9.
PMID: 31080012
13. Young J, Zastrow-Hayes G, Deschamps S, Svitashev S, Zaremba M, Acharya A, Paulraj S, Peterson-Burch B, Schwartz C, Djukanovic V, Lenderts B, Feigenbutz L, Wang L, Alarcon C, Siksnys V, May G, Chilcoat ND, Kumar S. CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement. Sci Rep. 2019 Apr 30;9(1):6729. doi: 10.1038/s41598-019-43141-6.
PMID: 31040331
14. Mogila I, Kazlauskiene M, Valinskyte S, Tamulaitiene G, Tamulaitis G, Siksnys V. Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits. Cell Rep. 2019 Mar 5;26(10):2753-2765.e4. doi: 10.1016/j.celrep.2019.02.029.
PMID: 30840895
15. Tamulaitiene G, Manakova E, Jovaisaite V, Tamulaitis G, Grazulis S, Bochtler M, Siksnys V. Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family. Nucleic Acids Res. 2019 Jan 25;47(2):997-1010. doi: 10.1093/nar/gky1137.
PMID: 30445642
16. Gordeeva J, Morozova N, Sierro N, Isaev A, Sinkunas T, Tsvetkova K, Matlashov M, Truncaite L, Morgan RD, Ivanov NV, Siksnys V, Zeng L, Severinov K. BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. Nucleic Acids Res. 2019 Jan 10;47(1):253-265. doi: 10.1093/nar/gky1125.
PMID: 30418590